Hotspot

ndhF

SSC · 109269-111494 · 2226 bp

Back to Ixeris

Pi 0.456
MarkerSeek score 77.50
Primer available yes
Label ndhF

Feature Metadata

label_name
ndhF
feature_type
gene
region
SSC
length_bp
2226
variable_sites
799
pi
0.456
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.79
markerseek_score
77.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF_p1 GGCCCTCCTATCTCTTCCTT AACGAGGTAAGATCCGGTGA 55.0 50.0 57.9 58.2 2655 bp 0.5 57.9
ndhF_p2 GGCCCTCCTATCTCTTCCTT ACGAGGTAAGATCCGGTGAA 55.0 50.0 57.9 58.2 2654 bp 0.5 57.9
ndhF_p3 CTAGCACTGGGTAGACCTCA AACGAGGTAAGATCCGGTGA 55.0 50.0 57.9 58.2 2515 bp 0.5 57.6
ndhF_p4 CTAGCACTGGGTAGACCTCA ACGAGGTAAGATCCGGTGAA 55.0 50.0 57.9 58.2 2514 bp 0.5 57.6
ndhF_p5 CCCTTCCATGCCTCATTTCA ACGAGGTAAGATCCGGTGAA 50.0 50.0 57.9 58.2 2988 bp 0.5 57.6