Hotspot

ndhD

SSC · 114945-116447 · 1503 bp

Pi 0.468

MarkerSeek score 81.70

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1503
variable_sites: 621
pi: 0.468
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 81.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	CGAGAACCGTGTGAATCACT	CTGCTGAAGCTGCTATTGGA	50.0	50.0	57.9	58.0	2383 bp	0.5	59.2
ndhD_p2	CGAGAACCGTGTGAATCACT	GCTGAAGCTGCTATTGGACT	50.0	50.0	57.9	58.0	2381 bp	0.5	59.2
ndhD_p3	CGAGAACCGTGTGAATCACT	TGATACCTTGGGACGGATGT	50.0	50.0	57.9	58.1	1970 bp	0.5	58.5
ndhD_p4	TCAGGAGCAGTATGGGCTAA	CTGCTGAAGCTGCTATTGGA	50.0	50.0	57.8	58.0	2706 bp	0.5	58.3
ndhD_p5	TTCAGGAGCAGTATGGGCTA	CTGCTGAAGCTGCTATTGGA	50.0	50.0	57.8	58.0	2707 bp	0.5	58.3