Hotspot

ndhH-rps15

SSC · 122338-122427 · 90 bp

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Pi 0.407
MarkerSeek score 75.90
Primer available yes
Label ndhH-rps15

Feature Metadata

label_name
ndhH-rps15
feature_type
igs
region
SSC
length_bp
90
variable_sites
33
pi
0.407
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.90
markerseek_score
75.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhH-rps15_p1 GGTCCTGACAAACCCCAATT AATAATGGGAGCCGCTTGAG 50.0 50.0 58.0 58.0 1479 bp 0.5 59.7
ndhH-rps15_p2 GTAAATCAGCAGCGACTCCA AATAATGGGAGCCGCTTGAG 50.0 50.0 58.0 58.0 1316 bp 0.5 59.7
ndhH-rps15_p3 TAAATCAGCAGCGACTCCAC AATAATGGGAGCCGCTTGAG 50.0 50.0 58.0 58.0 1315 bp 0.5 59.7
ndhH-rps15_p4 GGTCCTGACAAACCCCAATT GAATAATGGGAGCCGCTTGA 50.0 50.0 58.0 58.0 1480 bp 0.5 59.7
ndhH-rps15_p5 TAAATCAGCAGCGACTCCAC GAATAATGGGAGCCGCTTGA 50.0 50.0 58.0 58.0 1316 bp 0.5 59.7