Hotspot

ccsA-ndhD

SSC · 114686-114944 · 259 bp

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Pi 0.466
MarkerSeek score 76.30
Primer available yes
Label ccsA-ndhD

Feature Metadata

label_name
ccsA-ndhD
feature_type
igs
region
SSC
length_bp
259
variable_sites
110
pi
0.466
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.91
markerseek_score
76.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ccsA-ndhD_p1 ACCCAAGGCTTTGCTACTTC GTTTTTGATTCTGGACCGCG 50.0 50.0 58.1 58.0 1048 bp 0.5 59.0
ccsA-ndhD_p2 ACCCAAGGCTTTGCTACTTC GCTTGCATTACCGGGTATGA 50.0 50.0 58.1 58.0 1253 bp 0.5 59.0
ccsA-ndhD_p3 CGGCTCTTCTTTGTGGATCA GTTTTTGATTCTGGACCGCG 50.0 50.0 57.9 58.0 930 bp 0.5 58.9
ccsA-ndhD_p4 CGGCTCTTCTTTGTGGATCA GCTTGCATTACCGGGTATGA 50.0 50.0 57.9 58.0 1135 bp 0.5 58.9
ccsA-ndhD_p5 ACCCAAGGCTTTGCTACTTC TTCAACATCTCCTGGCCAAC 50.0 50.0 58.1 58.1 1532 bp 0.5 58.6