Hotspot

rps19

LSC · 85035-85313 · 279 bp

Back to Ivesia

Pi 0.0143
MarkerSeek score 56.80
Primer available yes
Label rps19

Feature Metadata

label_name
rps19
feature_type
gene
region
LSC
length_bp
279
variable_sites
4
pi
0.0143
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
56.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps19_p1 AGGTTCACCTCTCATTAATAAACGA TTGATTCTTCGTCGCCGTAG 36.0 50.0 58.1 58.1 456-478 bp 1.0 44.5
rps19_p2 GGTTCACCTCTCATTAATAAACGA TTGATTCTTCGTCGCCGTAG 37.5 50.0 56.8 58.1 455-477 bp 1.0 44.5
rps19_p3 AGGTTCACCTCTCATTAATAAACG TTGATTCTTCGTCGCCGTAG 37.5 50.0 56.8 58.1 456-478 bp 1.0 44.5
rps19_p4 AGGTTCACCTCTCATTAATAAACGA ATTTGATTCTTCGTCGCCGT 36.0 45.0 58.1 58.3 458-480 bp 1.0 44.4
rps19_p5 AGGTTCACCTCTCATTAATAAACGA TTTGATTCTTCGTCGCCGTA 36.0 45.0 58.1 57.6 457-479 bp 1.0 44.4