Hotspot

petD

LSC · 77461-78666 · 1206 bp

Pi 0.00419

MarkerSeek score 62.20

Primer available yes

Label petD

Feature Metadata

label_name: petD
feature_type: gene
region: LSC
length_bp: 1206
variable_sites: 5
pi: 0.00419
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD_p1	AGTTGAAGCGAATTCTCCGA	ACTTTGAAGCGATGATTCCCT	45.0	42.9	57.3	57.6	1300-1318 bp	1.0	64.6
petD_p2	AGTTGAAGCGAATTCTCCGA	CACTTTGAAGCGATGATTCCC	45.0	47.6	57.3	57.6	1301-1319 bp	1.0	64.2
petD_p3	TGAAGCGAATTCTCCGAAGA	ACTTTGAAGCGATGATTCCCT	45.0	42.9	57.0	57.6	1297-1315 bp	1.0	62.3
petD_p4	AGTTGAAGCGAATTCTCCGAA	ACTTTGAAGCGATGATTCCCT	42.9	42.9	58.0	57.6	1300-1318 bp	1.0	62.2
petD_p5	TGAAGCGAATTCTCCGAAGA	CACTTTGAAGCGATGATTCCC	45.0	47.6	57.0	57.6	1298-1316 bp	1.0	61.9