Hotspot

rpl32-trnL-UAG

SSC · 114871-115430 · 560 bp

Back to Ivesia

Pi 0.0268
MarkerSeek score 73.30
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
560
variable_sites
15
pi
0.0268
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
73.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 GGGCAGCATTGAAAGCTTTT AGCGTGTCTACCGATTTCAC 45.0 50.0 57.8 58.0 672-673 bp 1.0 80.3
rpl32-trnL-UAG_p2 GGGCAGCATTGAAAGCTTTT CAGCGTGTCTACCGATTTCA 45.0 50.0 57.8 58.0 673-674 bp 1.0 80.3
rpl32-trnL-UAG_p3 GGGCAGCATTGAAAGCTTTT TCCTAAGAGCAGCGTGTCTA 45.0 50.0 57.8 57.9 682-683 bp 1.0 79.4
rpl32-trnL-UAG_p4 GGGCAGCATTGAAAGCTTTT CCTAAGAGCAGCGTGTCTAC 45.0 55.0 57.8 57.8 681-682 bp 1.0 78.8
rpl32-trnL-UAG_p5 GGGCAGCATTGAAAGCTTTT CTAAGAGCAGCGTGTCTACC 45.0 55.0 57.8 57.8 680-681 bp 1.0 78.8