Hotspot

ndhF-rpl32

SSC · 113612-114711 · 1100 bp

Back to Ivesia

Pi 0.00371
MarkerSeek score 62.70
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
1100
variable_sites
4
pi
0.00371
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
62.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 AGGAACTGGAAGGGGAATGA AAAAGCTTTCAATGCTGCCC 50.0 45.0 57.9 57.8 1245-1253 bp 1.0 79.7
ndhF-rpl32_p2 AGGAACTGGAAGGGGAATGA GGAACTAGAGGTGCGCTTTT 50.0 50.0 57.9 58.2 1182-1190 bp 1.0 79.7
ndhF-rpl32_p3 AACTGGAAGGGGAATGAACG AAAAGCTTTCAATGCTGCCC 50.0 45.0 57.8 57.8 1242-1250 bp 1.0 78.6
ndhF-rpl32_p4 AACTGGAAGGGGAATGAACG GGAACTAGAGGTGCGCTTTT 50.0 50.0 57.8 58.2 1179-1187 bp 1.0 78.6
ndhF-rpl32_p5 TGGAAGGGGAATGAACGGTA AAAAGCTTTCAATGCTGCCC 50.0 45.0 58.3 57.8 1239-1247 bp 1.0 77.5