Hotspot

trnM-CAU-atpE

LSC · 53077-53257 · 181 bp

Back to Ivesia

Pi 0.0331
MarkerSeek score 64.60
Primer available yes
Label trnM-CAU-atpE

Feature Metadata

label_name
trnM-CAU-atpE
feature_type
igs
region
LSC
length_bp
181
variable_sites
6
pi
0.0331
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
64.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnM-CAU-atpE_p1 ATCCATCAATGCGACAGGTC ACTTGAGGAAAGCTGAAGGC 50.0 50.0 58.0 58.1 396-397 bp 1.0 80.1
trnM-CAU-atpE_p2 ATCCATCAATGCGACAGGTC CTTGAGGAAAGCTGAAGGCA 50.0 50.0 58.0 58.1 395-396 bp 1.0 80.1
trnM-CAU-atpE_p3 ATCCATCAATGCGACAGGTC TGAGGAAAGCTGAAGGCAAG 50.0 50.0 58.0 58.1 393-394 bp 1.0 80.1
trnM-CAU-atpE_p4 AATGCGACAGGTCCCATTTT ACTTGAGGAAAGCTGAAGGC 45.0 50.0 58.1 58.1 389-390 bp 1.0 79.8
trnM-CAU-atpE_p5 ATGCGACAGGTCCCATTTTT ACTTGAGGAAAGCTGAAGGC 45.0 50.0 58.1 58.1 388-389 bp 1.0 79.8