Hotspot

trnF-GAA-ndhJ

LSC · 49132-49850 · 719 bp

Back to Ivesia

Pi 0.00145
MarkerSeek score 63.30
Primer available yes
Label trnF-GAA-ndhJ

Feature Metadata

label_name
trnF-GAA-ndhJ
feature_type
igs
region
LSC
length_bp
719
variable_sites
1
pi
0.00145
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.96
markerseek_score
63.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnF-GAA-ndhJ_p1 GGGATAGCTCAGCTGGTAGA ATCCACGCCTGAAACGTATC 55.0 50.0 58.0 58.1 873-893 bp 1.0 82.1
trnF-GAA-ndhJ_p2 CCTCGTGTCACCAGTTCAAA ATCCACGCCTGAAACGTATC 50.0 50.0 58.1 58.1 837-857 bp 1.0 81.8
trnF-GAA-ndhJ_p3 GGGATAGCTCAGCTGGTAGA TGAAAGTTGGATAGGCTGGC 55.0 50.0 58.0 57.9 845-865 bp 1.0 81.7
trnF-GAA-ndhJ_p4 CCTCGTGTCACCAGTTCAAA TGAAAGTTGGATAGGCTGGC 50.0 50.0 58.1 57.9 809-829 bp 1.0 81.4
trnF-GAA-ndhJ_p5 GTCGTGAATGGTCGGGATAG ATCCACGCCTGAAACGTATC 55.0 50.0 57.9 58.1 886-906 bp 1.0 81.0