Hotspot

atpF-atpH

LSC · 12709-13229 · 521 bp

Back to Ivesia

Pi 0.00198
MarkerSeek score 61.40
Primer available yes
Label atpF-atpH

Feature Metadata

label_name
atpF-atpH
feature_type
igs
region
LSC
length_bp
521
variable_sites
1
pi
0.00198
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
61.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpF-atpH_p1 ATTAAACCCGAAACTCCCCG CGGAAGGAAAAATACGGGGT 50.0 50.0 57.9 57.9 686-703 bp 1.0 80.2
atpF-atpH_p2 ATTAAACCCGAAACTCCCCG CCAGAGGCGGAAGGAAAAAT 50.0 50.0 57.9 58.2 693-710 bp 1.0 79.8
atpF-atpH_p3 ATTAAACCCGAAACTCCCCG AGGCGGAAGGAAAAATACGG 50.0 50.0 57.9 58.3 689-706 bp 1.0 79.1
atpF-atpH_p4 GATGGCCAATAACCCAAGGA CGGAAGGAAAAATACGGGGT 50.0 50.0 57.5 57.9 664-681 bp 1.0 77.6
atpF-atpH_p5 GATGGCCAATAACCCAAGGA CCAGAGGCGGAAGGAAAAAT 50.0 50.0 57.5 58.2 671-688 bp 1.0 77.2