Hotspot

trnR-UCU-atpA

LSC · 9998-10579 · 582 bp

Back to Ivesia

Pi 0.00859
MarkerSeek score 57.10
Primer available yes
Label trnR-UCU-atpA

Feature Metadata

label_name
trnR-UCU-atpA
feature_type
igs
region
LSC
length_bp
582
variable_sites
5
pi
0.00859
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
57.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnR-UCU-atpA_p1 ATTGGAGTGAAAAGCGTCCA AGGAGCAAGTGGAACGTTTT 45.0 45.0 57.7 57.9 708 bp 1.0 78.0
trnR-UCU-atpA_p2 TGGAGTGAAAAGCGTCCATT AGGAGCAAGTGGAACGTTTT 45.0 45.0 57.7 57.9 706 bp 1.0 78.0
trnR-UCU-atpA_p3 TTGGAGTGAAAAGCGTCCAT AGGAGCAAGTGGAACGTTTT 45.0 45.0 57.7 57.9 707 bp 1.0 78.0
trnR-UCU-atpA_p4 TGGAGTGAAAAGCGTCCATT ATTCAGGAGCAAGTGGAACG 45.0 50.0 57.7 58.2 710 bp 1.0 76.9
trnR-UCU-atpA_p5 TTGGAGTGAAAAGCGTCCAT ATTCAGGAGCAAGTGGAACG 45.0 50.0 57.7 58.2 711 bp 1.0 76.9