Hotspot

psaC

SSC · 122480-122725 · 246 bp

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Pi 0.537
MarkerSeek score 75.30
Primer available yes
Label psaC

Feature Metadata

label_name
psaC
feature_type
gene
region
SSC
length_bp
246
variable_sites
116
pi
0.537
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.88
markerseek_score
75.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaC_p1 CGCGTGGAGTTTTCTGAAAC ATCTCTCATGAGCCGATCCA 50.0 50.0 58.0 58.0 421 bp 0.5 59.8
psaC_p2 TCGCGTGGAGTTTTCTGAAA ATCTCTCATGAGCCGATCCA 45.0 50.0 58.1 58.0 422 bp 0.5 59.5
psaC_p3 TTCGCGTGGAGTTTTCTGAA ATCTCTCATGAGCCGATCCA 45.0 50.0 58.1 58.0 423 bp 0.5 59.5
psaC_p4 GGACCAGAAAAACCCGTGAT ATCTCTCATGAGCCGATCCA 50.0 50.0 58.1 58.0 495 bp 0.5 59.2
psaC_p5 TTCCTCCCCACATAGCTAGG ATCTCTCATGAGCCGATCCA 55.0 50.0 57.9 58.0 987 bp 0.5 59.1