Hotspot

ndhG

SSC · 123561-124091 · 531 bp

Pi 0.453

MarkerSeek score 79.10

Primer available yes

Label ndhG

Feature Metadata

label_name: ndhG
feature_type: gene
region: SSC
length_bp: 531
variable_sites: 202
pi: 0.453
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.84
markerseek_score: 79.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG_p1	TGGATCGGCTCATGAGAGAT	ATCAAATCGCTTTGGGTCGT	50.0	45.0	58.0	58.2	1709 bp	0.5	58.5
ndhG_p2	TGGATCGGCTCATGAGAGAT	ACAACAGGATAAACCCCACG	50.0	50.0	58.0	57.8	1632 bp	0.5	58.4
ndhG_p3	TGGATCGGCTCATGAGAGAT	TCTACATCAACCCACACCCA	50.0	50.0	58.0	58.3	1396 bp	0.5	57.8
ndhG_p4	TGGATCGGCTCATGAGAGAT	ATTCGCCTCAAACAACAGGA	50.0	45.0	58.0	57.7	1643 bp	0.5	57.8
ndhG_p5	TGGATCGGCTCATGAGAGAT	TTCGCCTCAAACAACAGGAT	50.0	45.0	58.0	57.7	1642 bp	0.5	57.8