Hotspot

ndhA

SSC · 125053-127261 · 2209 bp

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Pi 0.461
MarkerSeek score 78.10
Primer available yes
Label ndhA

Feature Metadata

label_name
ndhA
feature_type
gene
region
SSC
length_bp
2209
variable_sites
821
pi
0.461
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.81
markerseek_score
78.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhA_p1 TGCAGCTCGTACTGTTTGTT AAGCACCAAAGGGAGAGTTG 45.0 50.0 58.1 58.0 2532 bp 0.5 59.5
ndhA_p2 TTGCAGCTCGTACTGTTTGT AAGCACCAAAGGGAGAGTTG 45.0 50.0 58.1 58.0 2533 bp 0.5 59.5
ndhA_p3 TGCAGCTCGTACTGTTTGTT TTAGAACGGGTTGAGGGAGT 45.0 50.0 58.1 58.0 2926 bp 0.5 59.4
ndhA_p4 TTGCAGCTCGTACTGTTTGT TTAGAACGGGTTGAGGGAGT 45.0 50.0 58.1 58.0 2927 bp 0.5 59.4
ndhA_p5 TGCAGCTCGTACTGTTTGTT GCATTATTGGCGGAGAGGAA 45.0 50.0 58.1 58.0 2904 bp 0.5 59.3