Hotspot

ccsA

SSC · 119712-120683 · 972 bp

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Pi 0.447
MarkerSeek score 81.50
Primer available yes
Label ccsA

Feature Metadata

label_name
ccsA
feature_type
gene
region
SSC
length_bp
972
variable_sites
378
pi
0.447
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.87
markerseek_score
81.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ccsA_p1 GTGAAATCGGTAGACACGCT CTCGACGAAATGGGTGGAAT 50.0 50.0 58.0 58.0 1731 bp 0.5 59.7
ccsA_p2 TGAAATCGGTAGACACGCTG CTCGACGAAATGGGTGGAAT 50.0 50.0 58.0 58.0 1730 bp 0.5 59.7
ccsA_p3 GTGAAATCGGTAGACACGCT TTATCGATGGCTTCCCTTGC 50.0 50.0 58.0 58.0 1671 bp 0.5 59.7
ccsA_p4 TGAAATCGGTAGACACGCTG TTATCGATGGCTTCCCTTGC 50.0 50.0 58.0 58.0 1670 bp 0.5 59.7
ccsA_p5 GGCGGCATGGAATCTTCTAA CTCGACGAAATGGGTGGAAT 50.0 50.0 58.0 58.0 1667 bp 0.5 59.5