Hotspot

ndhD-psaC

SSC · 122363-122479 · 117 bp

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Pi 0.491
MarkerSeek score 76.70
Primer available yes
Label ndhD-psaC

Feature Metadata

label_name
ndhD-psaC
feature_type
igs
region
SSC
length_bp
117
variable_sites
53
pi
0.491
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.92
markerseek_score
76.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhD-psaC_p1 GCTTCTCCATGAGTGTCTGG ATCTCTCATGAGCCGATCCA 55.0 50.0 58.0 58.0 1237 bp 0.5 59.8
ndhD-psaC_p2 TTCCTCCCCACATAGCTAGG ATCTCTCATGAGCCGATCCA 55.0 50.0 57.9 58.0 987 bp 0.5 59.1
ndhD-psaC_p3 GCTTCTCCATGAGTGTCTGG TGTGAATCTGCCTGTCCAAC 55.0 50.0 58.0 58.1 926 bp 0.5 59.0
ndhD-psaC_p4 GCTTCTCCATGAGTGTCTGG TGCTTCTGCTCCAAGAACAG 55.0 50.0 58.0 58.1 969 bp 0.5 58.9
ndhD-psaC_p5 GCTTCTCCATGAGTGTCTGG TGATACCTTGGGACGGATGT 55.0 50.0 58.0 58.1 1003 bp 0.5 58.8