Hotspot

ndhF-rpl32

SSC · 117602-118505 · 904 bp

Back to Itea

Pi 0.462
MarkerSeek score 81.00
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
904
variable_sites
359
pi
0.462
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.86
markerseek_score
81.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 TGTTACCGCCGGAAAAAGAA TGCTGCCCAATATCCCTTTC 45.0 50.0 58.0 57.9 1087 bp 0.5 59.1
ndhF-rpl32_p2 TTGTTACCGCCGGAAAAAGA TGCTGCCCAATATCCCTTTC 45.0 50.0 58.0 57.9 1088 bp 0.5 59.1
ndhF-rpl32_p3 GCCCACATACGACGAAGATT TGCTGCCCAATATCCCTTTC 50.0 50.0 58.1 57.9 1110 bp 0.5 58.9
ndhF-rpl32_p4 ACGAATGGGATGATCCATGC TGCTGCCCAATATCCCTTTC 50.0 50.0 58.1 57.9 1029 bp 0.5 58.6
ndhF-rpl32_p5 CGAATGGGATGATCCATGCA TGCTGCCCAATATCCCTTTC 50.0 50.0 58.1 57.9 1028 bp 0.5 58.6