Hotspot

psbC-trnS-UGA

LSC · 38618-38839 · 222 bp

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Pi 0.045
MarkerSeek score 76.20
Primer available yes
Label psbC-trnS-UGA

Feature Metadata

label_name
psbC-trnS-UGA
feature_type
igs
region
LSC
length_bp
222
variable_sites
10
pi
0.045
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
76.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbC-trnS-UGA_p1 CCTGTTCTTTCCATGACCCC GAGAGATGGCTGAGTGGTTG 55.0 55.0 58.2 58.0 343-345 bp 1.0 80.9
psbC-trnS-UGA_p2 CCTGTTCTTTCCATGACCCC GTCTTTCGGAGAGATGGCTG 55.0 55.0 58.2 58.1 351-353 bp 1.0 80.5
psbC-trnS-UGA_p3 TCGTAGGTCATTTGTGGCAC GAGAGATGGCTGAGTGGTTG 50.0 55.0 58.2 58.0 423-425 bp 1.0 80.1
psbC-trnS-UGA_p4 CCTGTTCTTTCCATGACCCC TCTTTCGGAGAGATGGCTGA 55.0 50.0 58.2 58.1 350-352 bp 1.0 79.8
psbC-trnS-UGA_p5 TCGTAGGTCATTTGTGGCAC GTCTTTCGGAGAGATGGCTG 50.0 55.0 58.2 58.1 431-433 bp 1.0 79.7