Hotspot

ycf2

Genome · 124570-130931 · 6362 bp

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Pi 0.0324

MarkerSeek score 66.80

Primer available yes

Label ycf2

Feature Metadata

label_name: ycf2
feature_type: gene
region: Genome
length_bp: 6362
variable_sites: 901
pi: 0.0324
unique_haplotype_count: 49
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 66.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf2_p1	ACGTCAAGCCATTCTTCGTA	GGCGACCGATACAGCTTTTA	45.0	50.0	57.3	58.1	759-822 bp	0.773585	76.5
ycf2_p2	TACGTCAAGCCATTCTTCGT	GGCGACCGATACAGCTTTTA	45.0	50.0	57.3	58.1	760-823 bp	0.773585	76.5
ycf2_p3	ACGTCAAGCCATTCTTCGTA	TTTTTCATACAGGAGGGCGG	45.0	50.0	57.3	57.9	795-858 bp	0.773585	76.1
ycf2_p4	TACGTCAAGCCATTCTTCGT	TTTTTCATACAGGAGGGCGG	45.0	50.0	57.3	57.9	796-859 bp	0.773585	76.1
ycf2_p5	ACGTCAAGCCATTCTTCGTA	AGATCCCTCCATCGACGAAA	45.0	50.0	57.3	58.2	490-532 bp	0.377358	59.4