Hotspot

trnP-GGG-rpl32

Genome · 117438-117798 · 361 bp

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Pi 0.0515
MarkerSeek score 73.70
Primer available yes
Label trnP-GGG-rpl32

Feature Metadata

label_name
trnP-GGG-rpl32
feature_type
igs
region
Genome
length_bp
361
variable_sites
77
pi
0.0515
unique_haplotype_count
40
primer_available
yes
alignment_reliability
1.00
markerseek_score
73.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnP-GGG-rpl32_p1 ATAGGGAAGGGTGACGGAAA GGAGGTGCAGTAGTGAAAGG 50.0 55.0 58.0 57.9 1367-1484 bp 0.622642 75.8
trnP-GGG-rpl32_p2 ATAGGGAAGGGTGACGGAAA AAGAATTCTGCAGTCCCGTC 50.0 50.0 58.0 57.9 1351-1464 bp 0.45283 69.0
trnP-GGG-rpl32_p3 ATAGGGAAGGGTGACGGAAA GAAGAATTCTGCAGTCCCGT 50.0 50.0 58.0 57.9 1352-1465 bp 0.45283 69.0
trnP-GGG-rpl32_p4 ATAGGGAAGGGTGACGGAAA GAATTCTGCAGTCCCGTCTT 50.0 50.0 58.0 57.9 1349-1462 bp 0.45283 69.0
trnP-GGG-rpl32_p5 ATAGGGAAGGGTGACGGAAA GGGAGGTGCAGTAGTGAAAG 50.0 55.0 58.0 57.9 1372-1485 bp 0.45283 69.0