Hotspot

ccsA-trnL-UAG

Genome · 116972-117158 · 187 bp

Back to Isoetes

Pi 0.0457

MarkerSeek score 67.00

Primer available yes

Label ccsA-trnL-UAG

Feature Metadata

label_name: ccsA-trnL-UAG
feature_type: igs
region: Genome
length_bp: 187
variable_sites: 36
pi: 0.0457
unique_haplotype_count: 26
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-trnL-UAG_p1	TGTTCCAAGGTTCTGAGTATCG	AAGCATCTCGGTTCGAATCC	45.5	50.0	57.8	58.1	227-272 bp	0.962264	72.5
ccsA-trnL-UAG_p2	GTTCCAAGGTTCTGAGTATCGT	AAGCATCTCGGTTCGAATCC	45.5	50.0	57.8	58.1	226-271 bp	0.962264	72.5
ccsA-trnL-UAG_p3	TGTTCCAAGGTTCTGAGTATCG	GCTAAAGCATCTCGGTTCGA	45.5	50.0	57.8	58.1	231-276 bp	0.962264	72.4
ccsA-trnL-UAG_p4	GTTCCAAGGTTCTGAGTATCGT	GCTAAAGCATCTCGGTTCGA	45.5	50.0	57.8	58.1	230-275 bp	0.962264	72.4
ccsA-trnL-UAG_p5	TGTTCCAAGGTTCTGAGTATCG	TAGACACGCTGCTCTTAGGA	45.5	50.0	57.8	57.9	257-302 bp	0.962264	72.0