Hotspot

psbK-psbI

Genome · 66114-66495 · 382 bp

Back to Isoetes

Pi 0.0343

MarkerSeek score 65.60

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: Genome
length_bp: 382
variable_sites: 60
pi: 0.0343
unique_haplotype_count: 40
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 65.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	CCTCCGTGAGTTTCCGATAA	ACCACTGTGTAAACGAGCAG	50.0	50.0	57.0	58.1	396-502 bp	0.981132	82.5
psbK-psbI_p2	CCTCCGTGAGTTTCCGATAA	GACCACTGTGTAAACGAGCA	50.0	50.0	57.0	58.1	397-503 bp	0.981132	82.5
psbK-psbI_p3	CCTCCGTGAGTTTCCGATAA	TGACCACTGTGTAAACGAGC	50.0	50.0	57.0	58.1	398-504 bp	0.981132	82.5
psbK-psbI_p4	CCTCCGTGAGTTTCCGATAA	TGTGTAAACGAGCAGCTTGA	50.0	45.0	57.0	57.8	391-497 bp	0.981132	81.8
psbK-psbI_p5	CCTCCGTGAGTTTCCGATAA	ACTGTGTAAACGAGCAGCTT	50.0	45.0	57.0	57.8	393-499 bp	0.981132	81.7