Hotspot

psbD-trnT-GGU

Genome · 54379-55711 · 1333 bp

Back to Isoetes

Pi 0.0307

MarkerSeek score 66.60

Primer available yes

Label psbD-trnT-GGU

Feature Metadata

label_name: psbD-trnT-GGU
feature_type: igs
region: Genome
length_bp: 1333
variable_sites: 178
pi: 0.0307
unique_haplotype_count: 34
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 66.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbD-trnT-GGU_p1	ACTTTCCAATGGCTATAGTCA	GTGGTAGAGTAACGCCATGG	38.1	55.0	54.3	58.1	1413-1512 bp	1.0	54.0
psbD-trnT-GGU_p2	ACTTTCCAATGGCTATAGTCA	TCAGTGGTAGAGTAACGCCA	38.1	50.0	54.3	58.1	1416-1515 bp	1.0	53.9
psbD-trnT-GGU_p3	AACTTTCCAATGGCTATAGTCA	GTGGTAGAGTAACGCCATGG	36.4	55.0	55.1	58.1	1414-1513 bp	1.0	52.6
psbD-trnT-GGU_p4	AACTTTCCAATGGCTATAGTCA	TCAGTGGTAGAGTAACGCCA	36.4	50.0	55.1	58.1	1417-1516 bp	1.0	52.5
psbD-trnT-GGU_p5	ACTTTCCAATGGCTATAGTCA	GGCGTAAGTCATCGGTTCAA	38.1	50.0	54.3	58.3	1390-1489 bp	1.0	52.3