Hotspot

rbcL-atpB

Genome · 32373-33203 · 831 bp

Back to Isoetes

Pi 0.0568

MarkerSeek score 74.60

Primer available yes

Label rbcL-atpB

Feature Metadata

label_name: rbcL-atpB
feature_type: igs
region: Genome
length_bp: 831
variable_sites: 214
pi: 0.0568
unique_haplotype_count: 37
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 74.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-atpB_p1	TCTCTCCAACGCATAAACGG	CGTCCAAGAGTAGCTTCACC	50.0	55.0	58.0	58.0	1787-1833 bp	1.0	91.9
rbcL-atpB_p2	TGACCCTTAATCTCACCCGT	CGTCCAAGAGTAGCTTCACC	50.0	55.0	58.1	58.0	1853-1899 bp	1.0	91.6
rbcL-atpB_p3	ACCTTCCTCGAACAGATCCA	CGTCCAAGAGTAGCTTCACC	50.0	55.0	58.1	58.0	1473-1519 bp	1.0	91.5
rbcL-atpB_p4	GCTTTGAATCCAACACTCGC	CGTCCAAGAGTAGCTTCACC	50.0	55.0	58.0	58.0	1184-1230 bp	0.830189	84.9
rbcL-atpB_p5	CGGTCTCTCCAACGCATAAA	CGTCCAAGAGTAGCTTCACC	50.0	55.0	58.0	58.0	1790-1836 bp	0.679245	79.1