Hotspot

petL-psbE

Genome · 21164-22978 · 1815 bp

Back to Isoetes

Pi 0.0324

MarkerSeek score 67.00

Primer available yes

Label petL-psbE

Feature Metadata

label_name: petL-psbE
feature_type: igs
region: Genome
length_bp: 1815
variable_sites: 264
pi: 0.0324
unique_haplotype_count: 34
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 67.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petL-psbE_p1	AAAGCTGGAGTTAGAGCAGC	CTCTCCCGTATTCCCAGACA	50.0	55.0	57.9	58.2	1420-1892 bp	1.0	89.4
petL-psbE_p2	AAAGCTGGAGTTAGAGCAGC	TCTCCCGTATTCCCAGACAT	50.0	50.0	57.9	57.5	1419-1891 bp	1.0	87.4
petL-psbE_p3	CTGGAGTTAGAGCAGCCAAT	CTCTCCCGTATTCCCAGACA	50.0	55.0	57.3	58.2	1416-1888 bp	1.0	84.6
petL-psbE_p4	AAAGCTGGAGTTAGAGCAGC	AATAATGTCGGCGAAAGGGC	50.0	50.0	57.9	59.0	1441-1913 bp	1.0	83.3
petL-psbE_p5	CTGGAGTTAGAGCAGCCAAT	TCTCCCGTATTCCCAGACAT	50.0	50.0	57.3	57.5	1415-1887 bp	1.0	82.6