Hotspot

psaJ-trnP-UGG

Genome · 19712-20380 · 669 bp

Back to Isoetes

Pi 0.0293

MarkerSeek score 67.20

Primer available yes

Label psaJ-trnP-UGG

Feature Metadata

label_name: psaJ-trnP-UGG
feature_type: igs
region: Genome
length_bp: 669
variable_sites: 85
pi: 0.0293
unique_haplotype_count: 37
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 67.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-trnP-UGG_p1	AGGCGCTGTGGAAAGATATG	CGAACTGAACTAAGAGCGCT	50.0	50.0	58.0	58.0	924-991 bp	1.0	91.5
psaJ-trnP-UGG_p2	CAGGCGCTGTGGAAAGATAT	CGAACTGAACTAAGAGCGCT	50.0	50.0	58.0	58.0	925-992 bp	1.0	91.5
psaJ-trnP-UGG_p3	CACGAACCTCCCCAATACTG	CGAACTGAACTAAGAGCGCT	55.0	50.0	58.0	58.0	1512-1578 bp	0.924528	88.7
psaJ-trnP-UGG_p4	CACGAACCTCCCCAATACTG	GGAGACCTACGTTCTACCGA	55.0	55.0	58.0	58.0	1529-1595 bp	0.924528	88.5
psaJ-trnP-UGG_p5	TCACGAACCTCCCCAATACT	CGAACTGAACTAAGAGCGCT	50.0	50.0	58.1	58.0	1513-1579 bp	0.924528	88.4