Hotspot

psbB-clpP

Genome · 13942-15056 · 1115 bp

Back to Isoetes

Pi 0.0295
MarkerSeek score 67.70
Primer available yes
Label psbB-clpP

Feature Metadata

label_name
psbB-clpP
feature_type
igs
region
Genome
length_bp
1115
variable_sites
145
pi
0.0295
unique_haplotype_count
40
primer_available
yes
alignment_reliability
0.99
markerseek_score
67.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbB-clpP_p1 ATACTCCAACCACCCCATGA AGTCGCACTGTACGTCAATC 50.0 50.0 58.0 58.0 993-1487 bp 1.0 91.7
psbB-clpP_p2 TGATACTCCAACCACCCCAT AGTCGCACTGTACGTCAATC 50.0 50.0 58.0 58.0 995-1489 bp 1.0 91.7
psbB-clpP_p3 GGTTGTGCTTCTCCAGTCAA AGTCGCACTGTACGTCAATC 50.0 50.0 58.0 58.0 1293-1787 bp 1.0 91.5
psbB-clpP_p4 TTGTGCTTCTCCAGTCAACC AGTCGCACTGTACGTCAATC 50.0 50.0 58.0 58.0 1291-1785 bp 1.0 91.5
psbB-clpP_p5 AGAAACTAACGCTGTGTGCA AGTCGCACTGTACGTCAATC 45.0 50.0 58.1 58.0 847-1341 bp 1.0 91.4