Hotspot

ycf1

SSC · 122416-127938 · 5523 bp

Pi 0.00842

MarkerSeek score 52.10

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5523
variable_sites: 471
pi: 0.00842
unique_haplotype_count: 82
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GACGAACTCGGAGATGCAAT	TTCGTTCAAGAAGGGGCAAA	50.0	45.0	58.1	57.9	1102-1153 bp	0.976744	87.0
ycf1_p2	GACGAACTCGGAGATGCAAT	AAGAAGGGGCAAACGTGTAG	50.0	50.0	58.1	58.1	1095-1146 bp	0.976744	86.7
ycf1_p3	GGGGTTGACCAAAAGGAAGT	TTCGTTCAAGAAGGGGCAAA	50.0	45.0	57.9	57.9	768-813 bp	0.860465	80.9
ycf1_p4	GGGGTTGACCAAAAGGAAGT	AAGAAGGGGCAAACGTGTAG	50.0	50.0	57.9	58.1	761-806 bp	0.848837	79.9
ycf1_p5	TCCATTCGGGAAGTTGCAAT	TTCGTTCAAGAAGGGGCAAA	45.0	45.0	57.8	57.9	519-543 bp	0.813953	76.8