Hotspot

psaC

SSC · 115797-116042 · 246 bp

Back to Isodon

Pi 0.00527
MarkerSeek score 28.50
Primer available yes
Label psaC

Feature Metadata

label_name
psaC
feature_type
gene
region
SSC
length_bp
246
variable_sites
8
pi
0.00527
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
28.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaC_p1 TGGATTCAAGTAGAGTTTTATGGA AGACCGAAAATTTATGACGTTCA 33.3 34.8 55.5 56.8 313 bp 0.94186 39.2
psaC_p2 TGGATTCAAGTAGAGTTTTATGGAA AGACCGAAAATTTATGACGTTCA 32.0 34.8 56.2 56.8 313 bp 0.94186 39.2
psaC_p3 TGGATTCAAGTAGAGTTTTATGGA AGACCGAAAATTTATGACGTTCAA 33.3 33.3 55.5 57.5 313 bp 0.94186 39.2
psaC_p4 TGGATTCAAGTAGAGTTTTATGGA GACCGAAAATTTATGACGTTCA 33.3 36.4 55.5 55.4 312 bp 0.94186 39.2
psaC_p5 TGGATTCAAGTAGAGTTTTATGG AGACCGAAAATTTATGACGTTCA 34.8 34.8 54.1 56.8 313 bp 0.94186 39.2