Hotspot

ndhF

SSC · 109272-111503 · 2232 bp

Back to Isodon

Pi 0.00632
MarkerSeek score 48.70
Primer available yes
Label ndhF

Feature Metadata

label_name
ndhF
feature_type
gene
region
SSC
length_bp
2232
variable_sites
161
pi
0.00632
unique_haplotype_count
66
primer_available
yes
alignment_reliability
1.00
markerseek_score
48.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF_p1 AAGGCGGATCCGAACAAAAT AACGCTGACCAATAGCCTTT 45.0 45.0 58.2 57.8 2945-2961 bp 0.872093 77.0
ndhF_p2 AAGACGATCCTCCTCCTTCC AACGCTGACCAATAGCCTTT 55.0 45.0 58.2 57.8 2980-2996 bp 0.872093 76.5
ndhF_p3 GACGATCCTCCTCCTTCCTT AACGCTGACCAATAGCCTTT 55.0 45.0 58.2 57.8 2978-2994 bp 0.872093 76.5
ndhF_p4 AAGGCGGATCCGAACAAAAT ACGCTGACCAATAGCCTTTC 45.0 50.0 58.2 58.3 2944-2960 bp 0.872093 76.5
ndhF_p5 ACGATCCTCCTCCTTCCTTT AACGCTGACCAATAGCCTTT 50.0 45.0 57.7 57.8 2977-2993 bp 0.872093 76.2