Hotspot

ccsA-ndhD

SSC · 113945-114176 · 232 bp

Back to Isodon

Pi 0.0356
MarkerSeek score 61.90
Primer available yes
Label ccsA-ndhD

Feature Metadata

label_name
ccsA-ndhD
feature_type
igs
region
SSC
length_bp
232
variable_sites
31
pi
0.0356
unique_haplotype_count
22
primer_available
yes
alignment_reliability
1.00
markerseek_score
61.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ccsA-ndhD_p1 TGGATATGCTATTTTGGAGTAA ACCCCGATTTCGTTCTTTCA 31.8 45.0 52.6 57.4 372-408 bp 1.0 51.8
ccsA-ndhD_p2 TGGATATGCTATTTTGGAGTAAA ACCCCGATTTCGTTCTTTCA 30.4 45.0 53.5 57.4 372-408 bp 1.0 51.8
ccsA-ndhD_p3 TGGATATGCTATTTTGGAGTAA CCCCGATTTCGTTCTTTCAC 31.8 50.0 52.6 57.1 371-407 bp 1.0 51.8
ccsA-ndhD_p4 TGGATATGCTATTTTGGAGTAAATCT ACCCCGATTTCGTTCTTTCA 30.8 45.0 56.5 57.4 372-408 bp 0.988372 51.3
ccsA-ndhD_p5 TGGATATGCTATTTTGGAGTAA TGGTATGTACCCCGATTTCG 31.8 50.0 52.6 57.1 380-416 bp 0.976744 50.0