Hotspot

rpl32-trnL-UAG

SSC · 112142-112906 · 765 bp

Back to Isodon

Pi 0.0147
MarkerSeek score 60.40
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
765
variable_sites
105
pi
0.0147
unique_haplotype_count
60
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 TCGTTGCTGTCGGAAAAAGA TCCTAAGAGCAGCGTGTCTA 45.0 50.0 58.1 57.9 1532-1556 bp 0.988372 87.9
rpl32-trnL-UAG_p2 TCGTTGCTGTCGGAAAAAGA AACCACTCGGATTGAAGAGC 45.0 50.0 58.1 57.9 2068-2096 bp 0.988372 87.5
rpl32-trnL-UAG_p3 TCGTTGCTGTCGGAAAAAGA AGCAGCGTGTCTACCAATTT 45.0 45.0 58.1 57.8 1525-1549 bp 0.988372 87.5
rpl32-trnL-UAG_p4 TCGTTGCTGTCGGAAAAAGA CCTAAGAGCAGCGTGTCTAC 45.0 55.0 58.1 57.8 1531-1555 bp 0.988372 87.4
rpl32-trnL-UAG_p5 TCGTTGCTGTCGGAAAAAGA CTAAGAGCAGCGTGTCTACC 45.0 55.0 58.1 57.8 1530-1554 bp 0.988372 87.4