Hotspot

trnF-GAA-ndhJ

LSC · 48041-48707 · 667 bp

Back to Isodon

Pi 0.0076
MarkerSeek score 62.80
Primer available yes
Label trnF-GAA-ndhJ

Feature Metadata

label_name
trnF-GAA-ndhJ
feature_type
igs
region
LSC
length_bp
667
variable_sites
52
pi
0.0076
unique_haplotype_count
34
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnF-GAA-ndhJ_p1 GGGATAGCTCAGCTGGTAGA GTTGGATAGGATGGCCCTTG 55.0 55.0 58.0 58.0 765-824 bp 1.0 89.2
trnF-GAA-ndhJ_p2 GAGGGTGGGATGCTACATTG GTTGGATAGGATGGCCCTTG 55.0 55.0 58.0 58.0 794-853 bp 1.0 89.1
trnF-GAA-ndhJ_p3 CCTCGTGTCACCAGTTCAAA GTTGGATAGGATGGCCCTTG 50.0 55.0 58.1 58.0 729-788 bp 1.0 89.1
trnF-GAA-ndhJ_p4 GGGATAGCTCAGCTGGTAGA AGTTGGATAGGATGGCCCTT 55.0 50.0 58.0 58.1 766-825 bp 1.0 88.6
trnF-GAA-ndhJ_p5 GAGGGTGGGATGCTACATTG AGTTGGATAGGATGGCCCTT 55.0 50.0 58.0 58.1 795-854 bp 1.0 88.5