Hotspot

trnT-GGU-psbD

LSC · 31127-32443 · 1317 bp

Back to Isodon

Pi 0.00612
MarkerSeek score 58.50
Primer available yes
Label trnT-GGU-psbD

Feature Metadata

label_name
trnT-GGU-psbD
feature_type
igs
region
LSC
length_bp
1317
variable_sites
85
pi
0.00612
unique_haplotype_count
51
primer_available
yes
alignment_reliability
1.00
markerseek_score
58.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGU-psbD_p1 GTGGTAGAGTAACGCCATGG ACAAAAACGAAACGGTCCCT 55.0 45.0 58.1 58.2 1458-1531 bp 1.0 84.0
trnT-GGU-psbD_p2 TCAGTGGTAGAGTAACGCCA ACAAAAACGAAACGGTCCCT 50.0 45.0 58.1 58.2 1461-1534 bp 1.0 83.8
trnT-GGU-psbD_p3 GTGGTAGAGTAACGCCATGG GCCAGACCAGCCTACAAAAA 55.0 50.0 58.1 58.4 1471-1544 bp 1.0 82.8
trnT-GGU-psbD_p4 GTGGTAGAGTAACGCCATGG CAAAAACGAAACGGTCCCTC 55.0 50.0 58.1 57.6 1457-1530 bp 1.0 82.8
trnT-GGU-psbD_p5 ACATAGTGACTCGTTCGGGA ACAAAAACGAAACGGTCCCT 50.0 45.0 58.2 58.2 1506-1579 bp 0.988372 82.5