Hotspot

petN-psbM

LSC · 28413-29404 · 992 bp

Back to Isodon

Pi 0.00939
MarkerSeek score 71.10
Primer available yes
Label petN-psbM

Feature Metadata

label_name
petN-psbM
feature_type
igs
region
LSC
length_bp
992
variable_sites
90
pi
0.00939
unique_haplotype_count
64
primer_available
yes
alignment_reliability
1.00
markerseek_score
71.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petN-psbM_p1 ACTCGTAGTGTGGGGAAGAA AGTTCCTACCGCTTTTCTACT 50.0 42.9 58.0 56.4 615-1098 bp 1.0 70.1
petN-psbM_p2 TCGTAGTGTGGGGAAGAAGT AGTTCCTACCGCTTTTCTACT 50.0 42.9 58.0 56.4 613-1096 bp 1.0 70.1
petN-psbM_p3 ACTCGTAGTGTGGGGAAGAA TCATTCTAGTTCCTACCGCT 50.0 45.0 58.0 55.4 622-1105 bp 1.0 69.9
petN-psbM_p4 TCGTAGTGTGGGGAAGAAGT TCATTCTAGTTCCTACCGCT 50.0 45.0 58.0 55.4 620-1103 bp 1.0 69.9
petN-psbM_p5 CGTAGTGTGGGGAAGAAGTG AGTTCCTACCGCTTTTCTACT 55.0 42.9 57.9 56.4 612-1095 bp 1.0 69.5