Hotspot

atpH-atpI

LSC · 13586-14586 · 1001 bp

Back to Isodon

Pi 0.00838
MarkerSeek score 69.30
Primer available yes
Label atpH-atpI

Feature Metadata

label_name
atpH-atpI
feature_type
igs
region
LSC
length_bp
1001
variable_sites
80
pi
0.00838
unique_haplotype_count
55
primer_available
yes
alignment_reliability
1.00
markerseek_score
69.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpH-atpI_p1 AACGGAAGCAGCAGAAATCA TCCCTGTCATGTTCCTTGGA 45.0 50.0 58.1 58.3 1064-1152 bp 1.0 87.0
atpH-atpI_p2 AGCCAATCCAGCAGCAATAA TCCCTGTCATGTTCCTTGGA 45.0 50.0 57.9 58.3 1082-1170 bp 1.0 86.5
atpH-atpI_p3 GCCAATCCAGCAGCAATAAC TCCCTGTCATGTTCCTTGGA 50.0 50.0 57.8 58.3 1081-1169 bp 1.0 86.0
atpH-atpI_p4 TCCAGCAGCAATAACGGAAG TCCCTGTCATGTTCCTTGGA 50.0 50.0 58.3 58.3 1076-1164 bp 1.0 85.7
atpH-atpI_p5 AACGGAAGCAGCAGAAATCA TTTTTGCAACTTTAGCCGCG 45.0 45.0 58.1 58.5 1016-1104 bp 1.0 85.2