Hotspot

OOC40_pgp086-trnQ-UUG

LSC · 5934-6894 · 961 bp

Back to Isodon

Pi 0.0103
MarkerSeek score 60.80
Primer available yes
Label OOC40_pgp086-trnQ-UUG

Feature Metadata

label_name
OOC40_pgp086-trnQ-UUG
feature_type
igs
region
LSC
length_bp
961
variable_sites
93
pi
0.0103
unique_haplotype_count
52
primer_available
yes
alignment_reliability
0.98
markerseek_score
60.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
OOC40_pgp086-trnQ-UUG_p1 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 794-1082 bp 0.988372 89.1
OOC40_pgp086-trnQ-UUG_p2 TATCCTTCAAGTCGCACGTT GAGGTTCGAATCCTTCCGTC 45.0 55.0 57.3 58.1 802-1090 bp 1.0 84.9
OOC40_pgp086-trnQ-UUG_p3 CGTTGCTTTCTACCACATCG GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.2 58.1 786-1074 bp 1.0 84.6
OOC40_pgp086-trnQ-UUG_p4 AAGTCGCACGTTGCTTTCTA TTCGGAGGTTCGAATCCTTC 45.0 50.0 58.1 57.3 798-1086 bp 0.988372 84.4
OOC40_pgp086-trnQ-UUG_p5 AAGTCGCACGTTGCTTTCTA TAAGGCAACGGGTTTTGGTC 45.0 50.0 58.1 58.7 824-1112 bp 0.988372 84.1