Hotspot

trnK-UUU-OOC40_pgp086

LSC · 4264-4787 · 524 bp

Back to Isodon

Pi 0.00947
MarkerSeek score 61.60
Primer available yes
Label trnK-UUU-OOC40_pgp086

Feature Metadata

label_name
trnK-UUU-OOC40_pgp086
feature_type
igs
region
LSC
length_bp
524
variable_sites
61
pi
0.00947
unique_haplotype_count
46
primer_available
yes
alignment_reliability
1.00
markerseek_score
61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-OOC40_pgp086_p1 TCTAGCCGCACTTAAAAGCC AAAAGGAGCTCAACCCACAG 50.0 50.0 58.3 58.0 638-680 bp 0.988372 89.0
trnK-UUU-OOC40_pgp086_p2 CCGCACTTAAAAGCCGAGTA AAAAGGAGCTCAACCCACAG 50.0 50.0 58.3 58.0 633-675 bp 0.988372 88.9
trnK-UUU-OOC40_pgp086_p3 TCGCGGTCTTCCAAAGTTTA AAAAGGAGCTCAACCCACAG 45.0 50.0 57.5 58.0 705-747 bp 0.988372 86.5
trnK-UUU-OOC40_pgp086_p4 TCTAGCCGCACTTAAAAGCC AAAAAGGAGCTCAACCCACA 50.0 45.0 58.3 57.5 639-681 bp 1.0 86.0
trnK-UUU-OOC40_pgp086_p5 CGCGGTCTTCCAAAGTTTAC AAAAGGAGCTCAACCCACAG 50.0 50.0 57.4 58.0 704-746 bp 0.988372 86.0