Hotspot

rps8

LSC · 77717-78127 · 411 bp

Back to Iseilema

Pi 0.00487

MarkerSeek score 32.80

Primer available yes

Label rps8

Feature Metadata

label_name: rps8
feature_type: gene
region: LSC
length_bp: 411
variable_sites: 5
pi: 0.00487
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 32.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps8_p1	TTGGGTACTATAGGTGCGGC	TGAGTAGATTGTGTCTCACGT	55.0	42.9	59.2	56.4	538 bp	1.0	50.3
rps8_p2	TTTGGGTACTATAGGTGCGG	TGAGTAGATTGTGTCTCACGT	50.0	42.9	56.7	56.4	539 bp	1.0	50.0
rps8_p3	GGTACTATAGGTGCGGCCA	TGAGTAGATTGTGTCTCACGT	57.9	42.9	58.6	56.4	535 bp	1.0	47.7
rps8_p4	TGGGTACTATAGGTGCGGC	TGAGTAGATTGTGTCTCACGT	57.9	42.9	58.6	56.4	537 bp	1.0	47.7
rps8_p5	TTGGGTACTATAGGTGCGGC	ATGAGTAGATTGTGTCTCACGT	55.0	40.9	59.2	56.9	539 bp	1.0	46.3