Hotspot

rps16

LSC · 4503-5598 · 1096 bp

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Pi 0.00191

MarkerSeek score 52.60

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1096
variable_sites: 6
pi: 0.00191
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	TGGCACGATATAAATGTAGCA	TCTCGATACCTTTTTGCAATGA	38.1	36.4	55.2	56.1	1243-1252 bp	1.0	40.8
rps16_p2	GGTACTAAGGACCCTCTCC	TCTCGATACCTTTTTGCAATGA	57.9	36.4	55.2	56.1	1174-1183 bp	1.0	40.8
rps16_p3	AGAAAGAGGCAAATTGTGTC	TCTCGATACCTTTTTGCAATGA	40.0	36.4	54.1	56.1	1207-1216 bp	1.0	40.8
rps16_p4	GGACTAAGAAAGAGGCAAATTGT	TCTCGATACCTTTTTGCAATGA	39.1	36.4	57.0	56.1	1213-1222 bp	1.0	40.8
rps16_p5	TGGCACGATATAAATGTAGCAA	TCTCGATACCTTTTTGCAATGA	36.4	36.4	56.0	56.1	1243-1252 bp	1.0	40.8