Hotspot

rpl32-trnL-UAG

SSC · 107672-108206 · 535 bp

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Pi 0.00908
MarkerSeek score 57.10
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
535
variable_sites
11
pi
0.00908
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
57.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 GGTTTTTCTGGGCAACAAGC TTCGAACCGAGATGCTTGAG 50.0 50.0 58.4 58.0 627-631 bp 1.0 79.4
rpl32-trnL-UAG_p2 GGTTTTTCTGGGCAACAAGC GGATTCGAACCGAGATGCTT 50.0 50.0 58.4 58.1 630-634 bp 1.0 79.0
rpl32-trnL-UAG_p3 GGTTTTTCTGGGCAACAAGC TCCTAAGAGCAGCGTGTCTA 50.0 50.0 58.4 57.9 600-604 bp 1.0 78.6
rpl32-trnL-UAG_p4 GGTTTTTCTGGGCAACAAGC AGCAGCGTGTCTACCAATTT 50.0 45.0 58.4 57.8 593-597 bp 1.0 78.1
rpl32-trnL-UAG_p5 GGTTTTTCTGGGCAACAAGC GCTTGAGCACTGCTTCCTAA 50.0 50.0 58.4 58.2 614-618 bp 1.0 78.0