Hotspot

rps12-trnV-GAC

IRb · 92394-94133 · 1740 bp

Back to Iseilema

Pi 0.0012

MarkerSeek score 55.20

Primer available yes

Label rps12-trnV-GAC

Feature Metadata

label_name: rps12-trnV-GAC
feature_type: igs
region: IRb
length_bp: 1740
variable_sites: 5
pi: 0.0012
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps12-trnV-GAC_p1	CGTAAGGCAGAGTTGGGTTT	GATGACTTCCACCACGTCAA	50.0	50.0	58.1	57.8	1805-1825 bp	1.0	78.9
rps12-trnV-GAC_p2	CGTAAGGCAGAGTTGGGTTT	AACTGATGACTTCCACCACG	50.0	50.0	58.1	57.8	1809-1829 bp	1.0	78.9
rps12-trnV-GAC_p3	CGTAAGGCAGAGTTGGGTTT	ATGACTTCCACCACGTCAAG	50.0	50.0	58.1	57.8	1804-1824 bp	1.0	78.9
rps12-trnV-GAC_p4	CGTAAGGCAGAGTTGGGTTT	TGGTGGGGGAGTAAGTCAAA	50.0	50.0	58.1	58.2	1870-1890 bp	1.0	78.8
rps12-trnV-GAC_p5	CGTAAGGCAGAGTTGGGTTT	CTGATGACTTCCACCACGTC	50.0	55.0	58.1	58.3	1807-1827 bp	1.0	78.0