Hotspot

ycf1

SSC · 123248-128557 · 5310 bp

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Pi 0.0198
MarkerSeek score 45.90
Primer available yes
Label ycf1

Feature Metadata

label_name
ycf1
feature_type
gene
region
SSC
length_bp
5310
variable_sites
303
pi
0.0198
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
45.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1_p1 AGCCCCCAAACCGTTAAAAA CGTTCCCAAAGGCGTAAAAC 45.0 50.0 58.2 58.0 608 bp 1.0 85.4
ycf1_p2 AGCCCCCAAACCGTTAAAAA GGATCTCTCGCGGAAGAATC 45.0 55.0 58.2 57.9 1192-1198 bp 1.0 85.0
ycf1_p3 AGTCGAGCCTTCTGAGATGA CGTTCCCAAAGGCGTAAAAC 50.0 50.0 57.9 58.0 554 bp 0.888889 80.8
ycf1_p4 AGTCGAGCCTTCTGAGATGA GGATCTCTCGCGGAAGAATC 50.0 55.0 57.9 57.9 1138-1144 bp 0.888889 80.2
ycf1_p5 AGTCGAGCCTTCTGAGATGA GCCAGGACTTCGTTCAAGAA 50.0 50.0 57.9 58.1 732 bp 0.666667 67.8