Hotspot

rpl32-trnL-UAG

SSC · 112991-113704 · 714 bp

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Pi 0.0474
MarkerSeek score 79.70
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
714
variable_sites
101
pi
0.0474
unique_haplotype_count
8
primer_available
yes
alignment_reliability
0.98
markerseek_score
79.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 TGGACATCGTTGAAAGCCTT TGCAGACTGAGGCATTTTGT 45.0 45.0 57.7 58.0 1347-1383 bp 1.0 88.8
rpl32-trnL-UAG_p2 TTGGACATCGTTGAAAGCCT TGCAGACTGAGGCATTTTGT 45.0 45.0 57.7 58.0 1348-1384 bp 1.0 88.8
rpl32-trnL-UAG_p3 TGGACATCGTTGAAAGCCTT TAACCACTGGGACTGGAGAG 45.0 55.0 57.7 58.1 1380-1416 bp 1.0 88.3
rpl32-trnL-UAG_p4 TGGACATCGTTGAAAGCCTT TGATCCACATAACAGAGCGC 45.0 50.0 57.7 58.1 1434-1470 bp 1.0 88.1
rpl32-trnL-UAG_p5 TTGGACATCGTTGAAAGCCT TGATCCACATAACAGAGCGC 45.0 50.0 57.7 58.1 1435-1471 bp 1.0 88.0