Hotspot

ndhF-rpl32

SSC · 112023-112831 · 809 bp

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Pi 0.0257
MarkerSeek score 62.00
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
809
variable_sites
62
pi
0.0257
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.99
markerseek_score
62.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 TCCATGAATATTGATATGTATGTTCCA ACGAATACGCTTTTTCGAGA 29.6 40.0 56.7 55.5 878-895 bp 1.0 47.7
ndhF-rpl32_p2 CCATGAATATTGATATGTATGTTCCA ACGAATACGCTTTTTCGAGA 30.8 40.0 55.5 55.5 877-894 bp 1.0 47.7
ndhF-rpl32_p3 TCCATGAATATTGATATGTATGTTCCA ACGAATACGCTTTTTCGAGAT 29.6 38.1 56.7 56.1 878-895 bp 1.0 47.7
ndhF-rpl32_p4 TCCATGAATATTGATATGTATGTTCC ACGAATACGCTTTTTCGAGA 30.8 40.0 55.3 55.5 878-895 bp 1.0 47.7
ndhF-rpl32_p5 CCATGAATATTGATATGTATGTTCCA ACGAATACGCTTTTTCGAGAT 30.8 38.1 55.5 56.1 877-894 bp 1.0 47.7