Hotspot

rpl36-rps8

LSC · 78930-79398 · 469 bp

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Pi 0.0268
MarkerSeek score 63.10
Primer available yes
Label rpl36-rps8

Feature Metadata

label_name
rpl36-rps8
feature_type
igs
region
LSC
length_bp
469
variable_sites
37
pi
0.0268
unique_haplotype_count
8
primer_available
yes
alignment_reliability
0.98
markerseek_score
63.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl36-rps8_p1 TTTTACGAACGGAAGCCCTT GGCGGAGAAATTTTGTGTTA 45.0 40.0 57.7 54.5 523-541 bp 1.0 57.0
rpl36-rps8_p2 ACGAACGGAAGCCCTTATTT GGCGGAGAAATTTTGTGTTA 45.0 40.0 57.5 54.5 519-537 bp 1.0 55.3
rpl36-rps8_p3 ATTTTACGAACGGAAGCCCT GGCGGAGAAATTTTGTGTTA 45.0 40.0 57.5 54.5 524-542 bp 1.0 55.2
rpl36-rps8_p4 TTTTACGAACGGAAGCCCTT GGCGGAGAAATTTTGTGTTAT 45.0 38.1 57.7 55.1 523-541 bp 1.0 53.9
rpl36-rps8_p5 ACGAACGGAAGCCCTTATTT GGCGGAGAAATTTTGTGTTAT 45.0 38.1 57.5 55.1 519-537 bp 1.0 52.1