Hotspot

psbE-petL

LSC · 63596-64959 · 1364 bp

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Pi 0.0168
MarkerSeek score 60.90
Primer available yes
Label psbE-petL

Feature Metadata

label_name
psbE-petL
feature_type
igs
region
LSC
length_bp
1364
variable_sites
66
pi
0.0168
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbE-petL_p1 ATAACCAGCCCGCAATGAAT AGCTGCTAGTAGAAAACCGA 45.0 45.0 57.9 56.3 1231-1682 bp 1.0 73.3
psbE-petL_p2 AGAACGTTCTCCTGTGCTTC AGCTGCTAGTAGAAAACCGA 50.0 45.0 57.8 56.3 1149-1600 bp 1.0 72.9
psbE-petL_p3 AATAACCAGCCCGCAATGAA AGCTGCTAGTAGAAAACCGA 45.0 45.0 58.2 56.3 1232-1683 bp 1.0 72.7
psbE-petL_p4 GTTCTCCTGTGCTTCCAGAC AGCTGCTAGTAGAAAACCGA 55.0 45.0 58.2 56.3 1144-1595 bp 1.0 72.5
psbE-petL_p5 ATAACCAGCCCGCAATGAAT AGCTGCTAGTAGAAAACCGAA 45.0 42.9 57.9 57.0 1231-1682 bp 1.0 71.4