Hotspot

petA-psbJ

LSC · 61855-62810 · 956 bp

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Pi 0.0218
MarkerSeek score 62.40
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
956
variable_sites
52
pi
0.0218
unique_haplotype_count
8
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 ATCCATTACGTGTCCAAGGC GAAGCGGGATAAATGGCTGA 50.0 50.0 58.0 58.0 1186-1231 bp 1.0 87.1
petA-psbJ_p2 ATCCATTACGTGTCCAAGGC AACAGGCCGTTTTGATCCTT 50.0 45.0 58.0 58.0 1639-1684 bp 1.0 86.4
petA-psbJ_p3 ATCCATTACGTGTCCAAGGC ACAGGCCGTTTTGATCCTTT 50.0 45.0 58.0 58.0 1638-1683 bp 1.0 86.4
petA-psbJ_p4 GTTTCAGAGGGCGAATCCAT AACAGGCCGTTTTGATCCTT 50.0 45.0 58.0 58.0 1736-1781 bp 1.0 86.1
petA-psbJ_p5 GTTTCAGAGGGCGAATCCAT ACAGGCCGTTTTGATCCTTT 50.0 45.0 58.0 58.0 1735-1780 bp 1.0 86.1