Hotspot

trnM-CAU-atpE

LSC · 51664-51800 · 137 bp

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Pi 0.0328
MarkerSeek score 61.10
Primer available yes
Label trnM-CAU-atpE

Feature Metadata

label_name
trnM-CAU-atpE
feature_type
igs
region
LSC
length_bp
137
variable_sites
12
pi
0.0328
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
61.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnM-CAU-atpE_p1 GGCAGGAGTCATTGGTTCAA AGTCGAGGCTCTCAATACGA 50.0 50.0 58.1 58.0 142-206 bp 1.0 88.1
trnM-CAU-atpE_p2 GGCAGGAGTCATTGGTTCAA ACGAGTCGAGGCTCTCAATA 50.0 50.0 58.1 58.0 145-209 bp 1.0 88.0
trnM-CAU-atpE_p3 TTGCTTTCATACGGCAGGAG AGTCGAGGCTCTCAATACGA 50.0 50.0 58.3 58.0 154-218 bp 1.0 86.4
trnM-CAU-atpE_p4 TTGCTTTCATACGGCAGGAG ACGAGTCGAGGCTCTCAATA 50.0 50.0 58.3 58.0 157-221 bp 1.0 86.3
trnM-CAU-atpE_p5 CATACGGCAGGAGTCATTGG AGTCGAGGCTCTCAATACGA 55.0 50.0 58.4 58.0 147-211 bp 1.0 85.4